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in-house oligo synthesizer mermade 6  (BioAutomation corporation)

 
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    BioAutomation corporation in-house oligo synthesizer mermade 6
    In House Oligo Synthesizer Mermade 6, supplied by BioAutomation corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/in-house+oligo+synthesizer+mermade+6/mermade+6+oligo+synthesizer/pmc06905585-27-18-23
    Average 90 stars, based on 1 article reviews
    in-house oligo synthesizer mermade 6 - by Bioz Stars, 2026-10
    90/100 stars

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    Modification:

    Article Title: m 6 A minimally impacts the structure, dynamics, and Rev ARM binding properties of HIV-1 RRE stem IIB
    Article Snippet: .. RREIIB and m 6 A modified RREIIB (RREIIB m6A68 and RREIIB m6A26m6A68 ) were chemically synthesized using an in-house oligo synthesizer (MerMade 6, BioAutomation) with solid-phase RNA synthesis using N-acetyl protected 2’-tBDSilyl-phosphoramidites (ChemGenes Corporation) and 1 μmol standard columns (1000 Å, BioAutomation) with 4,4’-dimethoxytrityl (DMT)-off synthesis followed by base and 2’-O deprotection (100 μmol DMSO, 125 μL TEA•3HF, heat at 65oC for 2.5hrs), and ethanol precipitation[ ]. ..

    Synthesized:

    Article Title: m 6 A minimally impacts the structure, dynamics, and Rev ARM binding properties of HIV-1 RRE stem IIB
    Article Snippet: .. RREIIB and m 6 A modified RREIIB (RREIIB m6A68 and RREIIB m6A26m6A68 ) were chemically synthesized using an in-house oligo synthesizer (MerMade 6, BioAutomation) with solid-phase RNA synthesis using N-acetyl protected 2’-tBDSilyl-phosphoramidites (ChemGenes Corporation) and 1 μmol standard columns (1000 Å, BioAutomation) with 4,4’-dimethoxytrityl (DMT)-off synthesis followed by base and 2’-O deprotection (100 μmol DMSO, 125 μL TEA•3HF, heat at 65oC for 2.5hrs), and ethanol precipitation[ ]. ..

    Article Title: Dynamic ensemble of HIV-1 RRE stem IIB reveals non-native conformations that disrupt the Rev-binding site
    Article Snippet: RREIIB, RREII and mutants (A68C-RREIIB, U72C-RREIIB, A68C-RREII, U72C-RREII and A68C/G50A/C69U-RREII) were prepared by in vitro transcription using T7 RNA polymerase (New England Biolabs Inc.), chemically synthesized DNA templates (Integrated DNA Technologies) with designed T7 promoter sequence (TTAATACGACTCACTATA) and uniformly 13 C/ 15 N-labeled nucleotides triphosphates (Cambridge Isotope Laboratories Inc.). .. RREIIB mutants (UUCG-RRE20, UUCG-G48A/G50U and m 3 U72-RREIIB) were synthesized using an in-house oligo synthesizer (MerMade 6, BioAutomation) with solid-phase RNA synthesis using N-acetyl protected 2′-tBDSilyl-phosphoramidites (ChemGenes Corporation) and 1 μmol standard columns (1000 Å, BioAutomation) with 4,4′-dimethoxytrityl (DMT)-off synthesis followed by base and 2′-O deprotection (100 μmol DMSO, 125 μl TEA•3HF) and ethanol precipitation. ..

    Article Title: Dynamic ensemble of HIV-1 RRE stem IIB reveals non-native conformations that disrupt the Rev binding site
    Article Snippet: RREIIB and RREII were prepared by in vitro transcription using T7 RNA polymerase (New England Biolabs Inc.), chemically synthesized DNA templates (Integrated DNA Technologies) with designed T7 promoter sequence (TTAATACGACTCACTATA) and uniformly 13 C/ 15 N-labeled nucleotides triphosphates (Cambridge Isotope Laboratories Inc.). .. RREIIB mutants (RRE20, G48A/G50U-RREIIB, A68C-RREIIB, U72C-RREIIB, A68C/G50A/C69U-RREIIB, and m 3 U72-RREIIB), and RREII mutants (A68C-RREII, U72C-RREII, A68C/G50A/C69U-RREII) were synthesized using an in-house oligo synthesizer (MerMade 6, BioAutomation) with solid-phase RNA synthesis using N-acetyl protected 2’-tBDSilyl-phosphoramidites (ChemGenes Corporation) and 1 μmol standard columns (1000 Å, BioAutomation) with 4,4’-dimethoxytrityl (DMT)-off synthesis followed by base and 2’-O deprotection (100 μmol DMSO, 125 μL TEA•3FH), and ethanol precipitation. ..

    Ethanol Precipitation:

    Article Title: m 6 A minimally impacts the structure, dynamics, and Rev ARM binding properties of HIV-1 RRE stem IIB
    Article Snippet: .. RREIIB and m 6 A modified RREIIB (RREIIB m6A68 and RREIIB m6A26m6A68 ) were chemically synthesized using an in-house oligo synthesizer (MerMade 6, BioAutomation) with solid-phase RNA synthesis using N-acetyl protected 2’-tBDSilyl-phosphoramidites (ChemGenes Corporation) and 1 μmol standard columns (1000 Å, BioAutomation) with 4,4’-dimethoxytrityl (DMT)-off synthesis followed by base and 2’-O deprotection (100 μmol DMSO, 125 μL TEA•3HF, heat at 65oC for 2.5hrs), and ethanol precipitation[ ]. ..

    Article Title: Dynamic ensemble of HIV-1 RRE stem IIB reveals non-native conformations that disrupt the Rev-binding site
    Article Snippet: RREIIB, RREII and mutants (A68C-RREIIB, U72C-RREIIB, A68C-RREII, U72C-RREII and A68C/G50A/C69U-RREII) were prepared by in vitro transcription using T7 RNA polymerase (New England Biolabs Inc.), chemically synthesized DNA templates (Integrated DNA Technologies) with designed T7 promoter sequence (TTAATACGACTCACTATA) and uniformly 13 C/ 15 N-labeled nucleotides triphosphates (Cambridge Isotope Laboratories Inc.). .. RREIIB mutants (UUCG-RRE20, UUCG-G48A/G50U and m 3 U72-RREIIB) were synthesized using an in-house oligo synthesizer (MerMade 6, BioAutomation) with solid-phase RNA synthesis using N-acetyl protected 2′-tBDSilyl-phosphoramidites (ChemGenes Corporation) and 1 μmol standard columns (1000 Å, BioAutomation) with 4,4′-dimethoxytrityl (DMT)-off synthesis followed by base and 2′-O deprotection (100 μmol DMSO, 125 μl TEA•3HF) and ethanol precipitation. ..

    Article Title: Dynamic ensemble of HIV-1 RRE stem IIB reveals non-native conformations that disrupt the Rev binding site
    Article Snippet: RREIIB and RREII were prepared by in vitro transcription using T7 RNA polymerase (New England Biolabs Inc.), chemically synthesized DNA templates (Integrated DNA Technologies) with designed T7 promoter sequence (TTAATACGACTCACTATA) and uniformly 13 C/ 15 N-labeled nucleotides triphosphates (Cambridge Isotope Laboratories Inc.). .. RREIIB mutants (RRE20, G48A/G50U-RREIIB, A68C-RREIIB, U72C-RREIIB, A68C/G50A/C69U-RREIIB, and m 3 U72-RREIIB), and RREII mutants (A68C-RREII, U72C-RREII, A68C/G50A/C69U-RREII) were synthesized using an in-house oligo synthesizer (MerMade 6, BioAutomation) with solid-phase RNA synthesis using N-acetyl protected 2’-tBDSilyl-phosphoramidites (ChemGenes Corporation) and 1 μmol standard columns (1000 Å, BioAutomation) with 4,4’-dimethoxytrityl (DMT)-off synthesis followed by base and 2’-O deprotection (100 μmol DMSO, 125 μL TEA•3FH), and ethanol precipitation. ..



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